当前位置: 网站首页 >> 成果一览 >> 重要论文 >> 正文

Overexpression of geranyl diphosphate synthase small subunit 1 (LcGPPS.SSU1) enhances the monoterpene content and biomass

[发表时间]:2020-06-16 [拟稿人]: [审核人]: [责任编辑]:亚热带林业研究所 [浏览次数]:

论文题目:Overexpression ofgeranyl diphosphate synthase small subunit 1(LcGPPS.SSU1) enhances the monoterpene content and biomass

论文作者:Yunxiao Zhao, Yicun Chen, Ming Gao, Hengfu Yin, Liwen Wu*, Yangdong Wang*

期刊来源:Industrial Crops and Products

论文摘要:

Litsea cubeba(Lour.) Person, an economically important aromatic plant producing essential oils, has lemon-like fragrance and 96.44–98.44% monoterpene contents. Understanding the rate-limiting enzymes in the biosynthesis of monoterpenes inL. cubebaserves the purpose of designing of new approaches to metabolic engineering. In this study, we investigated the function ofgeranyl diphosphate synthase small subunit 1(LcGPPS.SSU1), a branchpoint gene of monoterpenes biosynthesis pathway inL. cubeba. The expression analysis showed thatLcGPPS.SSU1was highly expressed in fruits, where the essential oil was produced and stored, and induced by light and methyl jasmonate (MeJA). In addition, the promoter ofLcGPPS.SSU1could drive the trichome-specific expression in tobacco. Overexpression ofLcGPPS.SSU1enhanced the monoterpene production inL. cubebaand tobacco (Nicotiana benthamiana). Moreover, analysing the expression level analysis of the key genes, especially1- deoxy-D-xylulose 5- phosphate synthase(DXS), involved in mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathway showed the metabolic flux was increased by positive feedback regulation mechanism inLcGPPS.SSU1-overexpressing lines. Meanwhile, when MEP pathway flux increased, the seed yield, leaf area, plant height ofLcGPPS.SSU1-overexpressing lines were enhanced by elevating the MEP-derived primary metabolites, such as gibberellin (GA), due to the increased expression levels ofgeranylgeranyl diphosphate synthases 2(GGPPS2),GGPPS4andGA20-oxidase(GA20ox). Taken together, our results suggest thatLcGPPS.SSU1plays a vital role in monoterpenes biosynthesis.

论文链接:https://doi.org/10.1016/j.indcrop.2019.111926