论文题目： METHODS TO PROMOTE FRUIT FORMATION AND ASYMBIOTIC SEED GERMINATION OF CALANTHE TSOONGIANA T. TANG ET F. T. WANG

论文作者：Cai Xia Wang, Min Tian*, Ya Ting Jiang, and Ying Zhang

期刊来源： Propagation Of Ornamental Plants

卷(期)：2018， 18(2): 49-57

论文摘要：

Calanthe tsoongianaT. Tang et F.T. Wang, a terrestrial orchid that is endemic to China, has become rare and endangered due to limited natural reproduction and habitat destruction. There is an urgent need to conserve this species, while it has been poorly studied. Here we describe methods to promote fruit formation and asymbiotic seed germination of C. tsoongiana. The fruit set of C. tsoongiana was only 2.5% in the natural habitats. Artificial pollination resulted in 100% of capsule formation and all capsules survived to full maturity by bagging treatment. The seeds collected at 150 days after pollination were the optimum for asymbiotic germination. Among the six basal media tested, the highest germination percentage and the earliest initiation of germination were observed on VW (32.3%, 50.5 days) and half-strength MS (32.9%, 50.2 days). No significant effect existed among three temperatures (15°C, 20 °C and 25°C), while a 16 h photoperiod of 20 μmol m-2 s-1 was better than a 16 h photoperiod of 56 μmol m-2 s-1 or continuous darkness for seed germination and seedling formation of C. tsoongiana. Pretreatment with 1% sodium hypochlorite (NaOCl) for 40-60 min was effective inadvancing the initial of germination and improving the germination percentage. The methods described here will facilitate the propagation of C. tsoongiana, which may also contribute to its conservation, horticultural production and breeding.