论文题目：SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

论文作者：Shuangshuang Chen, Miao Yu, He Li，Ying Wang，Zhuchou Lu，Yunxing Zhang，Mingying Liu，Guirong Qiao，Longhua Wu，Xiaojiao Han，Renying Zhuo

期刊来源：ORIGINAL RESEARCH ARTICLE

论文摘要：

  The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup fromSedum alfredii. Designated asSaHsfA4c, the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression ofSaHsfA4cin yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcriptInSaHsfA4cwhich contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function ofSaHsfA4cunder Cd stress was characterized in transgenicArabidopsisand non-hyperaccumulation ecotypeS. alfredii.SaHsfA4cwas able to rescue the Cd sensitivity of theArabidopsis athsfa4cmutant.SaHsfA4creduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes andHspsin transgenic lines. The present results suggest thatSaHsfA4cincreases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression ofHsps.

论文链接：https://www.frontiersin.org/articles/10.3389/fpls.2020.00142/full